Application Methods
MT2 is an endogenous protein. The "Application Methods" primarily refer to its in vitro application guidelines in scientific research and biomedical experiments:
Reconstitution and Preparation: Recombinant MT2 protein is usually provided as a lyophilized powder. Before use, briefly centrifuge to allow the powder to settle at the bottom of the tube, then reconstitute with sterile deionized water to a working concentration of 0.1-1.0 mg/mL.
Storage and Aliquoting: To ensure protein activity, it is recommended to add 5%-50% glycerol as a preservative and immediately aliquot into small volumes. The working solution can be stored at 4°C for a short period (not exceeding one week). Long-term storage requires -20°C or -80°C.
Contraindications: Strictly avoid repeated freeze-thaw cycles to prevent protein degradation or aggregation and inactivation.
Application Scenarios
MT2 is primarily used as a key research reagent and biomarker in the following basic and clinical translational research:
* **Toxicology and Environmental Medicine:** To investigate the cytotoxic mechanisms caused by heavy metal (e.g., cadmium, mercury, lead) exposure, and to assess heavy metal detoxification pathways and bioaccumulation effects.
* **Immunology and Inflammation Research:** To explore the pathophysiological role of MT2 in regulating immune cell (e.g., CD4+ T cell) activation, oxidative stress signaling, and autoimmune diseases (e.g., experimental autoimmune encephalomyelitis).
* **Neuroscience Research:** As a neuroprotective factor, to study its expression changes and repair mechanisms after cerebral ischemia, neurodegenerative diseases, or central nervous system injury.
* **In vitro Cell Experiments:** As an additive or intervention target, to study the regulation of cell proliferation, apoptosis, and gene expression by metal ion homeostasis in cell culture experiments.
Storage Conditions
Unreconstituted lyophilized powder: Store in its original bottle, protected from light and moisture, at -20°C for long-term storage (several years); or refrigerate at 2–8°C (stable for approximately 6 months); room temperature 15–25°C is only suitable for the shipping window (several weeks). Do not repeatedly freeze and thaw.
Reconstituted solution: Store at 2–8°C, protected from light (wrapped in foil), and use within 4–6 weeks; research grade can be aliquoted and frozen at -20°C (3–4 months), but not recommended for clinical non-standard formulations. Discard the reconstituted solution if it becomes cloudy/discolored/contains particles.
FAQ
Q: What is the minimum order quantity?
A: The minimum order quantity is 10 bottles/set.
Q: How long does it take to ship?
A: In-stock orders will be shipped within 24 hours.
Q: What purity levels can you provide?
A: Standard purity 99%.
Q: Which courier service do you use?
A: FedEx, DHL, UPS, and postal services are available. Q: How long does shipping take?
A: Approximately 10-15 days.
Q: Can you provide a COA (Certificate of Account) quality inspection report and HPLC (High-Performance Liquid Chromatography) test report?
A: Yes, all goods undergo professional testing before shipment.
Q: Do you support OEM branding?
A: OEM branding service is supported, subject to minimum order quantity requirements.
Q: How is after-sales service handled?
A: After the customer signs for the goods, if it is confirmed that the quality problem is caused by our production, they can enjoy free replacement or refund. We adhere to a pragmatic and responsible service principle, actively respond to and properly handle all kinds of issues, without shirking responsibility or avoiding problems, and do our best to protect the rights and interests of our customers.
Q: What payment methods are available?
A: We support bank transfer, Bitcoin, Transferwise, and Xoom.
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