Application Methods
The application methods for LL-37 must be strictly standardized according to the product form (peptide raw material, ELISA kit, etc.) and application scenario. The core operations are as follows:
Peptide Raw Material Handling: As a research-grade peptide, it must be handled in a professional laboratory environment. Before use, it must be accurately dissolved in a suitable solvent (such as sterile water or buffer) under sterile conditions according to experimental requirements. It is recommended to complete the experiment on the same day after dissolution. If aliquoting is required, it should be aliquoted into small portions to avoid repeated freeze-thaw cycles and ensure activity stability.
ELISA Kit Operation: Taking the human bactericidal peptide LL-37 ELISA kit as an example, the double-antibody sandwich method is used for detection. The core procedure is as follows: Add the standard and the test sample (serum, plasma, cell culture supernatant, etc.) to the antibody-coated ELISA plate and incubate at 37°C in the dark for 1 hour; after washing the plate, add the biotin-labeled detection antibody and incubate at 37°C in the dark for 1 hour; after washing the plate again, add the enzyme conjugate and react at 37°C in the dark for 30 minutes; add TMB chromogenic solution and develop color in the dark; finally, add the stop solution and measure the absorbance at 450nm using an ELISA reader within 5 minutes. Calculate the sample concentration using a standard curve. Cross-contamination must be avoided during the operation. Replace pipette tips frequently, ensure uninterrupted reagent addition, and strictly control the incubation time and temperature.
Application Scenarios:
Based on existing research, the exploration of LL-37 mainly focuses on the following areas:
Anti-infection research:
Bacterial infection: Intervention research on multiple drug-resistant strains and biofilm-related infections.
Viral infections: Research on the antiviral mechanisms of viruses such as RSV and influenza A.
Fungal infections: Research on the inhibitory effects on opportunistic pathogens such as Candida albicans.
Immune regulation and inflammation research: LL-37, as a bridging molecule between innate and adaptive immunity, has been used to study its mechanism of action in inflammatory diseases such as psoriasis and periodontitis. It should be noted that LL-37 concentrations as high as 300 µM were detected in psoriatic lesions, while approximately 1 µM was found in the gingival crevicular fluid of periodontitis, suggesting that high concentrations of LL-37 may participate in the pathological process of the disease through cytotoxicity.
Wound Healing and Tissue Repair: Bioactive wound dressings containing LL-37 are one of the application areas currently being explored.
Storage Conditions:
LL-37 storage requires strict control based on product form to ensure activity and stability. Key requirements are as follows:
Peptide Raw Material Storage: Unopened lyophilized powder should be freeze-dried at -20°C and stored away from light in vacuum-sealed aluminum foil bags for long-term storage. Unused product after opening should be sealed promptly to prevent moisture absorption and should be used as soon as possible to prevent activity degradation.
Dissolved Product Storage:
If the dissolved LL-37 solution cannot be used on the same day, it should be aliquoted and stored at -20°C. It can be stored for approximately 30 days. After 30 days, activity should be carefully evaluated before use to avoid experimental failure due to degradation.
ELISA Kit Storage: Unopened kits should be refrigerated at 2-8℃ and remain stable within their shelf life. Unused strips, standards, and reagents after opening should be sealed and returned to their original packaging, also stored at 2-8℃, avoiding repeated freeze-thaw cycles and contamination.
General Storage Requirements: All LL-37 products must be stored in a dry, cool, and dark environment, away from heat sources, strong light, and humidity to prevent product deterioration. These products are for research use only and are strictly prohibited from clinical treatment, human use, or non-research applications. Handling and storage must comply with laboratory safety management regulations.
FAQ
Q: What is the minimum order quantity?
A: The minimum order quantity is 10 vials/set.
Q: How long does it take to ship?
A: In-stock orders will be shipped within 24 hours.
Q: What purity levels can you provide?
A: Standard purity 99%.
Q: Which courier service do you use?
A: FedEx, DHL, UPS, and postal services are available.
Q: How long does shipping take? A: Approximately 10-15 days.
Q: Can you provide a COA quality inspection report and HPLC test report?
A: Yes, all goods undergo professional testing before shipment.
Q: Do you support OEM branding?
A: OEM branding service is supported, subject to minimum order quantity requirements.
Q: How is after-sales service handled?
A: After the customer signs for the goods, if it is confirmed that the quality problem is caused by our production, they can enjoy free replacement or refund. We adhere to a pragmatic and responsible service principle, actively respond to and properly handle all kinds of issues, without shirking responsibility or avoiding problems, and do our best to protect the rights and interests of our customers.
Q: What payment methods are available?
A: We support bank transfer, Bitcoin, Transferwise, and Xoom.
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