Application Scenarios
Muscle Biology Research: Investigating the inhibitory effect of GDF-8 on myoblast proliferation and differentiation, exploring the molecular mechanisms of diseases such as muscle atrophy and muscular dystrophy.
Metabolic Disease Research: Investigating the regulatory role of GDF-8 in lipid and glucose metabolism, and its association with obesity and metabolic syndrome.
Drug Screening and Development: As a target molecule, used to screen or evaluate antagonists, antibodies, or small molecule compounds targeting the GDF-8/myosin pathway.
Stem Cell Research: Used to study the regulatory effects of GDF-8 on the myogenic differentiation of mesenchymal stem cells, and its role in the stem cell microenvironment.
Regenerative Medicine: Used in muscle tissue engineering and cell therapy research, exploring strategies to promote muscle regeneration and repair.
Storage and Usage Methods
Proper storage and handling are crucial to maintaining the bioactivity of GDF-8 protein. Please strictly follow the following guidelines:
1. Dosage Form and State
This product is a lyophilized powder filtered through a 0.2 μm filter under sterile conditions.
2. Storage Conditions
Lyophilized Powder: Recommended for long-term storage at -20℃ to -80℃, it can be stably stored for 2 years. It can be stably stored for short-term storage at room temperature for 3 weeks.
Reconstituted Solution: In a buffer solution containing carrier proteins (such as 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), it can be stored at 2-8℃ for 2-7 days. For longer-term storage, it is recommended to aliquot and freeze at -20℃ to -80℃, where it can be stably stored for 3-6 months.
3. Reconstitution Procedure (Critical Step)
Centrifugation: Before opening, centrifuge the lyophilized tubes in a high-speed centrifuge (approximately 10,000 rpm or 12,000 rpm for 30 seconds, or 3,000-3,500 rpm for 5 minutes) to allow any lyophilized powder accumulated on the tube walls or cap to settle to the bottom, minimizing powder loss.
Aseptic Dissolution: Redissolve using sterile deionized water (ddH₂O). A resolution concentration of at least 100 μg/mL is recommended. After adding water, avoid vortexing or vigorous blowing. Instead, use gentle shaking, slow inversion, or low-speed rocking to aid dissolution and prevent protein denaturation.
Dilution and Aliquoting: After thorough dissolution, dilute to the working concentration (recommended at least 10 μg/mL) with a buffer containing the carrier protein, according to subsequent experimental requirements. Aliquot in single-use quantities, avoiding repeated freeze-thaw cycles. Aliquot volume should be at least 10 μL.
Why Choose Us?
In the procurement of critical research raw materials, choosing a reliable supplier is crucial to ensuring the accuracy of experimental data and the smooth progress of projects. As a professional source manufacturer, we have the following irreplaceable advantages:
Source Manufacturer, Controllable Costs: We are a direct R&D and production base, eliminating intermediate distribution links and providing you with highly competitive prices, especially suitable for long-term, large-volume procurement needs.
Professional Equipment, Leading Technology: We possess a mature and advanced protein expression and purification platform (covering prokaryotic and eukaryotic systems), equipped with professional production equipment to ensure precise and controllable production parameters for each batch of products.
Stable Supply, Ample Production Capacity: As the original supplier, we have large-scale production capabilities, ensuring a continuous and stable supply of products, eliminating your concerns about supply cycles during your experiments.
High Purity, Superior Quality: Our products undergo rigorous purification processes, with purity exceeding 95% as verified by SDS-PAGE. Endotoxin levels, as detected by LAL method, are < 1 EU/μg, ensuring high biological activity (e.g., ED₅₀ value determined by MPC-11 cell proliferation inhibition assay).
After-Sales Support, Worry-Free Technical Support: We provide professional technical support and a comprehensive after-sales service system, committed to product quality, and providing full-process support from consultation to application.
FAQ
Q: Where are your products manufactured?
A: Our products are from Shenzhen, China.
Q: What is the minimum order quantity? A: Minimum order quantity is 10 bottles/set.
Q: How long does it take to ship?
A: In-stock orders will be shipped within 24 hours.
Q: What purity level can you provide?
A: Standard purity 99%.
Q: Which courier service do you use?
A: FedEx, DHL, UPS, and postal services are available.
Q: How long does shipping take?
A: Approximately 10-15 days.
Q: Can you provide a COA (Certificate of Analysis) report and HPLC (High-Performance Computing) report?
A: Yes, all goods undergo professional testing before shipment.
Q: Do you support OEM/ODM?
A: OEM/ODM service is supported, subject to minimum order quantity requirements.
Q: What are your after-sales service details?
A: After the customer receives the goods, if the quality problem is confirmed to be caused by our production, they can enjoy free replacement or refund. We adhere to a pragmatic and responsible service principle, actively responding to and properly handling all kinds of issues, without shirking responsibility or avoiding problems, and fully protecting customer rights.
Q: What payment methods are available?
A: We support bank transfers, Bitcoin, Transferwise, and Xoom.
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