Application Scenarios
* **Myosin Pathway Mechanism Research:** As an ActRIIB decoy receptor tool protein, it is used in in vitro cell experiments of the TGF-β family signaling pathway to compare phenotypic changes in cells after neutralization of different ligands and elucidate the regulatory mechanisms of muscle growth.
* **Muscle Atrophy Animal Model Research:** Animal models of Duchenne muscular dystrophy (DMD), age-related sarcopenia, and cachexia-related muscle wasting are used to observe lean body mass, muscle fiber cross-sectional area, and related indicators of motor function. This direction was also the original target for clinical development, and all valid data come from animals and terminated early clinical samples.
* **Muscle Injury Repair Research:** Animal models of skeletal muscle contusion and strain are used to observe muscle regeneration, satellite cell activation, and tissue remodeling processes, while simultaneously assessing changes in vascular system-related indicators.
* **Bone Metabolism Research:** The ActRIIB pathway is also involved in bone remodeling. Changes in bone mineral density can be observed in animal experiments, providing a fundamental understanding of the muscle-bone linkage mechanism.
Tool protein control experiments: Parallel experimental groups are often set up with Follistatin 288 and Follistatin 344 to compare the differences in in vivo efficacy caused by different ligand capture profiles. This is a high-frequency control protocol for myostatin.
Research Usage Methods
Reconstitution Procedure:
ACE-031 is an Fc fusion macromolecular protein, and its conformation is much more sensitive to the environment than that of ordinary small peptides.
After removing the lyophilized vials from cryogenic storage, allow them to fully warm to room temperature before opening the rubber stopper to avoid condensation and protein denaturation. Use the manufacturer's recommended sterile buffer solution for reconstitution; direct use of pure water is not recommended as it will directly disrupt the protein's spatial conformation.
Protect from light throughout the procedure. Minimize the room temperature operation time. Avoid vigorous shaking; dissolve the powder by slowly rotating the vial. The appearance of foam indicates protein denaturation. After reconstitution, aliquot according to the amount used in a single experiment to minimize repeated freeze-thaw cycles and exposure to air.
Storage Conditions
Unreconstituted Lyophilized Powder
Long-term Storage: Store at -20℃, sealed and protected from light, with a desiccant to prevent moisture absorption. Under these conditions, it can maintain biological activity for a relatively long time.
Short-term Use: Refrigerate at 2-8℃, protected from light. Suitable for raw materials that need to be used in experiments within a few weeks. Do not store at room temperature; high temperatures will directly cause irreversible inactivation of the fusion protein. If the powder clumps or changes color, it is considered protein denaturation and should not be used in experiments.
Reconstituted Protein Working Solution
Reconstituted protein solutions have poor stability. They should be aliquoted and stored frozen at -20℃ or -80℃. Storage at -80℃ has a longer shelf life; at -20℃, it is recommended to use it within one month. The number of freeze-thaw cycles should be strictly controlled to a maximum of one. Repeated freeze-thaw cycles directly destroy the dimer structure of the Fc fusion protein, significantly reducing its biological activity.
If temporarily refrigerated at 2-8℃, experiments must be completed within 48 hours. If the solution becomes cloudy or develops flocculent precipitates, discard it immediately.
FAQ
Q: What is the minimum order quantity? A: Minimum order quantity is 10 bottles/set.
Q: How long does it take to ship?
A: In-stock orders will be shipped within 24 hours.
Q: What purity level can you provide?
A: Standard purity 99%.
Q: Which courier service do you use?
A: FedEx, DHL, UPS, and postal services are available.
Q: How long does shipping take?
A: Approximately 10-15 days.
Q: Can you provide a COA (Certificate of Analysis) report and HPLC (High-Performance Computing) report?
A: Yes, all goods undergo professional testing before shipment.
Q: Do you support OEM/ODM?
A: OEM/ODM service is supported, subject to minimum order quantity requirements.
Q: What are your after-sales service details?
A: After the customer receives the goods, if the quality problem is confirmed to be caused by our production, they can enjoy free replacement or refund. We adhere to a pragmatic and responsible service principle, actively responding to and properly handling all kinds of issues, without shirking responsibility or avoiding problems, and fully protecting customer rights.
Q: What payment methods are available?
A: We support bank transfers, Bitcoin, Transferwise, and Xoom.
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