Application Scenarios
Body Composition Optimization and Lipid Metabolism
The core application of AOD-9604 is selective adipose tissue regulation. Unlike GLP-1 receptor agonists (such as smegglutinin) which act through central appetite suppression, AOD-9604 acts directly on the β3-AR of adipocytes, promoting lipolysis without affecting eating behavior. This difference in mechanism makes it suitable for the following populations: those who have completed a GLP-1 treatment course and entered a plateau or have discontinued the treatment, those who cannot tolerate the gastrointestinal side effects of GLP-1, and individuals who wish to maximize the retention of lean body mass during fat loss.
Metabolic Syndrome and Nonalcoholic Fatty Liver Disease
Based on its non-inducing insulin resistance characteristic, AOD-9604 has theoretical value in the study of metabolic syndrome and NAFLD. Animal models show that it can reduce total lipid mass and improve energy balance, but indications for metabolic diseases in humans have not yet been established.
Joint and Cartilage Repair
A 2015 study using a rabbit knee osteoarthritis model showed that intra-articular injection of AOD-9604 combined with hyaluronic acid improved cartilage histological scores and reduced lameness, with better results than hyaluronic acid alone. This evidence is limited to a single animal experiment; human joint indications are still in the conceptual stage.
### Research Reagent Usage
In vitro and in vivo studies of AOD9604 typically involve the reconstitution, administration, and detection of recombinant peptides. Specific procedures must strictly adhere to the following guidelines:
1. **Reconstitution and Storage of Lyophilized Powder**: Commercially available AOD9604 is mostly in the form of trifluoroacetate lyophilized powder. Before use, a brief centrifugation is necessary to allow the powder to settle at the bottom of the tube. It is recommended to use sterile physiological saline or PBS buffer for reconstitution, with a concentration generally not lower than 1 mg/mL. The reconstituted peptides readily adsorb onto the tube walls; therefore, it is recommended to use low-adsorption centrifuge tubes for aliquoting and store at -20°C. Repeated freeze-thaw cycles are strictly prohibited.
2. **In vivo administration regimen:** In animal experiments, AOD9604 is typically administered subcutaneously or intraperitoneally. For mouse models, the usual dose is 0.1-1 mg/kg/day for 4-8 weeks; for rat models, the dose can be adjusted to 0.5-2 mg/kg/day. Administration is usually scheduled before the start of the dark cycle (simulating the active metabolic period at night) to match the secretion rhythm of endogenous growth hormone.
3. **In vitro cell experiments:** In the 3T3-L1 preadipocyte differentiation model, the usual concentration of AOD9604 is 10-100 nM. Note that AOD9604 has a more significant lipolytic effect on mature adipocytes. Therefore, it is recommended to add it after cell differentiation is complete (approximately day 8-10), and continue treatment for 48-72 hours before detecting glycerol release and the expression of fatty acid oxidation-related genes (such as CPT1 and ACOX1).
Storage Conditions
Unopened lyophilized powder: Sealed and protected from light, 2–8 ℃ for short-term storage (several months to 1 year), -20 ℃ for standard long-term storage (12–24 months), -80 ℃ can further extend shelf life; transport on blue ice.
Reconstituted solution: 2–8 ℃ protected from light, research grade, routinely used within 2–4 weeks (some suppliers provide up to 28 days); strictly prohibit freeze-thaw cycles-although disulfide-bonded cyclic peptides are more stable than linear peptides, freeze-thaw cycles will still promote aggregation and precipitation.
Aliquot: Single-use, low-adsorption tubes, reconstitute without vortexing, discard immediately if flocculent/discoloration/phase separation occurs.
FAQ
Q: What is the minimum order quantity? A: Minimum order quantity is 10 bottles/set.
Q: How long does it take to ship?
A: In-stock orders will be shipped within 24 hours.
Q: What purity level can you provide?
A: Standard purity 99%.
Q: Which courier service do you use?
A: FedEx, DHL, UPS, and postal services are available.
Q: How long does shipping take?
A: Approximately 10-15 days.
Q: Can you provide a COA (Certificate of Analysis) report and HPLC (High-Performance Computing) report?
A: Yes, all goods undergo professional testing before shipment.
Q: Do you support OEM/ODM?
A: OEM/ODM service is supported, subject to minimum order quantity requirements.
Q: What are your after-sales service details?
A: After the customer receives the goods, if the quality problem is confirmed to be caused by our production, they can enjoy free replacement or refund. We adhere to a pragmatic and responsible service principle, actively responding to and properly handling all kinds of issues, without shirking responsibility or avoiding problems, and fully protecting customer rights.
Q: What payment methods are available?
A: We support bank transfers, Bitcoin, Transferwise, and Xoom.
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